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mouse monoclonal antibodies to either p53 (clone d01  (Merck KGaA)

 
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    Structured Review

    Merck KGaA mouse monoclonal antibodies to either p53 (clone d01
    ( A, D, G and J ) mRNA expression of <t>p53,</t> Mdm2, p21 and Bax respectively, in placental lysates at delivery. ( B, E, H, and K ) Protein expression of p53, Mdm2, p21 and Bax, respectively, through densitometry standardized to Myosin Light Chain (MLC) (*p<0.05, **p<0.01, n = 8). ( C, F, I, and L ) Localisation of p53, Mdm2, p21 and Bax respectively, in placental villi by immunohistochemistry. ST = Syncytiotrophoblast. Images counterstained with haematoxylin. Scale bar = 5 µm.
    Mouse Monoclonal Antibodies To Either P53 (Clone D01, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+antibodies+to+either+p53+(clone+d01/p53+antibody/pmc03907567-85-30-33
    Average 90 stars, based on 1 article reviews
    mouse monoclonal antibodies to either p53 (clone d01 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast"

    Article Title: Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087621

    ( A, D, G and J ) mRNA expression of p53, Mdm2, p21 and Bax respectively, in placental lysates at delivery. ( B, E, H, and K ) Protein expression of p53, Mdm2, p21 and Bax, respectively, through densitometry standardized to Myosin Light Chain (MLC) (*p<0.05, **p<0.01, n = 8). ( C, F, I, and L ) Localisation of p53, Mdm2, p21 and Bax respectively, in placental villi by immunohistochemistry. ST = Syncytiotrophoblast. Images counterstained with haematoxylin. Scale bar = 5 µm.
    Figure Legend Snippet: ( A, D, G and J ) mRNA expression of p53, Mdm2, p21 and Bax respectively, in placental lysates at delivery. ( B, E, H, and K ) Protein expression of p53, Mdm2, p21 and Bax, respectively, through densitometry standardized to Myosin Light Chain (MLC) (*p<0.05, **p<0.01, n = 8). ( C, F, I, and L ) Localisation of p53, Mdm2, p21 and Bax respectively, in placental villi by immunohistochemistry. ST = Syncytiotrophoblast. Images counterstained with haematoxylin. Scale bar = 5 µm.

    Techniques Used: Expressing, Immunohistochemistry

    ( A ) Transfection of placental explants with fluorescent labelled non-silencing siRNA demonstrating the presence of siRNA in cytotrophoblast (CT) and syncytiotrophoblast (ST). ( B and C ) Quantitative PCR demonstrating significant reductions in p53 and Mdm2 respectively, in explants treated with p53 or Mdm2 siRNA (*p< 0.05, **p<0.01, n = 6). ( D and E ) Western blot densitometry and immunohistochemistry showing a reduction in Mdm2 protein following treatment with Mdm2 siRNA. p53 expression is increased in trophoblast cytoplasm and nuclei in response to Mdm2 siRNA. ( F and G ) TUNEL index and images respectively, showing apoptosis significantly increased in explants exposed to Mdm2 siRNA (*p<0.05). Elevated apoptosis confirmed by M30 immunostaining with examples imaged by electron microscopy. ( H ) Proliferative index and representative images showing significantly reduced proliferation in explants cultured with Mdm2 siRNA (*p<0.05). ( I ) density of syncytial nuclear aggregates (SNA) increased by treatment Mdm2 siRNA (*p<0.05), representative images of control and Mdm2 siRNA (arrow = SNA). Representative images shown (FC = fetal capillary, IVS = intervillous space, CT = cytotrophoblast, ST = syncytiotrophoblast). All scale bars = 10 µm.
    Figure Legend Snippet: ( A ) Transfection of placental explants with fluorescent labelled non-silencing siRNA demonstrating the presence of siRNA in cytotrophoblast (CT) and syncytiotrophoblast (ST). ( B and C ) Quantitative PCR demonstrating significant reductions in p53 and Mdm2 respectively, in explants treated with p53 or Mdm2 siRNA (*p< 0.05, **p<0.01, n = 6). ( D and E ) Western blot densitometry and immunohistochemistry showing a reduction in Mdm2 protein following treatment with Mdm2 siRNA. p53 expression is increased in trophoblast cytoplasm and nuclei in response to Mdm2 siRNA. ( F and G ) TUNEL index and images respectively, showing apoptosis significantly increased in explants exposed to Mdm2 siRNA (*p<0.05). Elevated apoptosis confirmed by M30 immunostaining with examples imaged by electron microscopy. ( H ) Proliferative index and representative images showing significantly reduced proliferation in explants cultured with Mdm2 siRNA (*p<0.05). ( I ) density of syncytial nuclear aggregates (SNA) increased by treatment Mdm2 siRNA (*p<0.05), representative images of control and Mdm2 siRNA (arrow = SNA). Representative images shown (FC = fetal capillary, IVS = intervillous space, CT = cytotrophoblast, ST = syncytiotrophoblast). All scale bars = 10 µm.

    Techniques Used: Transfection, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Expressing, TUNEL Assay, Immunostaining, Electron Microscopy, Cell Culture

    ( A ) Representative Western Blots of control and Nutlin-3 treated normal placental lysates. Densitometry revealed a significant increase in the expression of ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma (*p<0.05, n = 5). ( F ) There was no effect on Bax. Co-treatment of BeWo cells with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) reduced caspase-3/7 activity ( G ) and TUNEL staining ( H ) to the level of controls. Representative images of TUNEL staining in ( I ) control, ( J ) Nutlin-3 and ( K ) co-treatment with Nutlin-3 and Pifithrin-α. Blue = DAPI, Green = TUNEL. Scale bar = 50 µm.
    Figure Legend Snippet: ( A ) Representative Western Blots of control and Nutlin-3 treated normal placental lysates. Densitometry revealed a significant increase in the expression of ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma (*p<0.05, n = 5). ( F ) There was no effect on Bax. Co-treatment of BeWo cells with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) reduced caspase-3/7 activity ( G ) and TUNEL staining ( H ) to the level of controls. Representative images of TUNEL staining in ( I ) control, ( J ) Nutlin-3 and ( K ) co-treatment with Nutlin-3 and Pifithrin-α. Blue = DAPI, Green = TUNEL. Scale bar = 50 µm.

    Techniques Used: Western Blot, Expressing, Activity Assay, TUNEL Assay, Staining

    ( A ) Western Blots of Nutlin-3 treated BeWo cell lysates and co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) demonstrated no effect upon ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma or ( F ) Bax protein expression (n = 5).
    Figure Legend Snippet: ( A ) Western Blots of Nutlin-3 treated BeWo cell lysates and co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) demonstrated no effect upon ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma or ( F ) Bax protein expression (n = 5).

    Techniques Used: Western Blot, Expressing

    Co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) had no effect on ( A ) p53, ( B ) Mdm2 or ( E ) Bax mRNA expression. Treatment with Nutlin-3 increased ( C ) p21 and ( D ) Puma expression an effect lost by co-treatment with Pifithrin-α (*p<0.05, **p<0.01, n = 5).
    Figure Legend Snippet: Co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) had no effect on ( A ) p53, ( B ) Mdm2 or ( E ) Bax mRNA expression. Treatment with Nutlin-3 increased ( C ) p21 and ( D ) Puma expression an effect lost by co-treatment with Pifithrin-α (*p<0.05, **p<0.01, n = 5).

    Techniques Used: Expressing



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    mouse monoclonal antibodies to either p53 (clone d01  (Merck KGaA)
    90
    Merck KGaA mouse monoclonal antibodies to either p53 (clone d01
    ( A, D, G and J ) mRNA expression of <t>p53,</t> Mdm2, p21 and Bax respectively, in placental lysates at delivery. ( B, E, H, and K ) Protein expression of p53, Mdm2, p21 and Bax, respectively, through densitometry standardized to Myosin Light Chain (MLC) (*p<0.05, **p<0.01, n = 8). ( C, F, I, and L ) Localisation of p53, Mdm2, p21 and Bax respectively, in placental villi by immunohistochemistry. ST = Syncytiotrophoblast. Images counterstained with haematoxylin. Scale bar = 5 µm.
    Mouse Monoclonal Antibodies To Either P53 (Clone D01, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+antibodies+to+either+p53+(clone+d01/p53+antibody/pmc03907567-85-30-33
    Average 90 stars, based on 1 article reviews
    mouse monoclonal antibodies to either p53 (clone d01 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A, D, G and J ) mRNA expression of p53, Mdm2, p21 and Bax respectively, in placental lysates at delivery. ( B, E, H, and K ) Protein expression of p53, Mdm2, p21 and Bax, respectively, through densitometry standardized to Myosin Light Chain (MLC) (*p<0.05, **p<0.01, n = 8). ( C, F, I, and L ) Localisation of p53, Mdm2, p21 and Bax respectively, in placental villi by immunohistochemistry. ST = Syncytiotrophoblast. Images counterstained with haematoxylin. Scale bar = 5 µm.

    Journal: PLoS ONE

    Article Title: Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast

    doi: 10.1371/journal.pone.0087621

    Figure Lengend Snippet: ( A, D, G and J ) mRNA expression of p53, Mdm2, p21 and Bax respectively, in placental lysates at delivery. ( B, E, H, and K ) Protein expression of p53, Mdm2, p21 and Bax, respectively, through densitometry standardized to Myosin Light Chain (MLC) (*p<0.05, **p<0.01, n = 8). ( C, F, I, and L ) Localisation of p53, Mdm2, p21 and Bax respectively, in placental villi by immunohistochemistry. ST = Syncytiotrophoblast. Images counterstained with haematoxylin. Scale bar = 5 µm.

    Article Snippet: Membranes were blocked for 1 hr with 3% (w/v) milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBS-T) and then probed overnight at 4°C, with mouse monoclonal antibodies to either p53 (Clone D01, Merck Biosciences, Nottingham, UK, 1 µg/ml (Explants), 0.1 µg/ml (BeWo)), Mdm2 (Clone 2A10, Merck Biosciences, 2 µg/ml), anti-p21 (Clone EA10, Merck Biosciences, 1∶100), Bak (TC-102, Merck Biosciences, 1∶200), Bcl-2 (Clone 100/D5, Abcam, Cambridge, UK, 1 µg/ml), Procaspase-3 (Clone 84803, R&D Systems, Abingdon, UK, 1∶1000), Procaspase 8 (Clone 84131, Merck Biosciences, 1∶100), Myosin Light Chain (Clone MY21, Abcam, 0.1 µg/ml), β-actin, (Clone AC15, Sigma, 1∶10,000) or rabbit polyclonal antibody against Bax (ab7977, Abcam, 1 µg/ml (Explants), 0.2 µg/ml (BeWo)), p21 (Abcam, 0.2 µg/ml (BeWo)), Puma (Abcam, 4 µg/ml) or β-actin (Clone AC15, Sigma, 1∶10,000).

    Techniques: Expressing, Immunohistochemistry

    ( A ) Transfection of placental explants with fluorescent labelled non-silencing siRNA demonstrating the presence of siRNA in cytotrophoblast (CT) and syncytiotrophoblast (ST). ( B and C ) Quantitative PCR demonstrating significant reductions in p53 and Mdm2 respectively, in explants treated with p53 or Mdm2 siRNA (*p< 0.05, **p<0.01, n = 6). ( D and E ) Western blot densitometry and immunohistochemistry showing a reduction in Mdm2 protein following treatment with Mdm2 siRNA. p53 expression is increased in trophoblast cytoplasm and nuclei in response to Mdm2 siRNA. ( F and G ) TUNEL index and images respectively, showing apoptosis significantly increased in explants exposed to Mdm2 siRNA (*p<0.05). Elevated apoptosis confirmed by M30 immunostaining with examples imaged by electron microscopy. ( H ) Proliferative index and representative images showing significantly reduced proliferation in explants cultured with Mdm2 siRNA (*p<0.05). ( I ) density of syncytial nuclear aggregates (SNA) increased by treatment Mdm2 siRNA (*p<0.05), representative images of control and Mdm2 siRNA (arrow = SNA). Representative images shown (FC = fetal capillary, IVS = intervillous space, CT = cytotrophoblast, ST = syncytiotrophoblast). All scale bars = 10 µm.

    Journal: PLoS ONE

    Article Title: Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast

    doi: 10.1371/journal.pone.0087621

    Figure Lengend Snippet: ( A ) Transfection of placental explants with fluorescent labelled non-silencing siRNA demonstrating the presence of siRNA in cytotrophoblast (CT) and syncytiotrophoblast (ST). ( B and C ) Quantitative PCR demonstrating significant reductions in p53 and Mdm2 respectively, in explants treated with p53 or Mdm2 siRNA (*p< 0.05, **p<0.01, n = 6). ( D and E ) Western blot densitometry and immunohistochemistry showing a reduction in Mdm2 protein following treatment with Mdm2 siRNA. p53 expression is increased in trophoblast cytoplasm and nuclei in response to Mdm2 siRNA. ( F and G ) TUNEL index and images respectively, showing apoptosis significantly increased in explants exposed to Mdm2 siRNA (*p<0.05). Elevated apoptosis confirmed by M30 immunostaining with examples imaged by electron microscopy. ( H ) Proliferative index and representative images showing significantly reduced proliferation in explants cultured with Mdm2 siRNA (*p<0.05). ( I ) density of syncytial nuclear aggregates (SNA) increased by treatment Mdm2 siRNA (*p<0.05), representative images of control and Mdm2 siRNA (arrow = SNA). Representative images shown (FC = fetal capillary, IVS = intervillous space, CT = cytotrophoblast, ST = syncytiotrophoblast). All scale bars = 10 µm.

    Article Snippet: Membranes were blocked for 1 hr with 3% (w/v) milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBS-T) and then probed overnight at 4°C, with mouse monoclonal antibodies to either p53 (Clone D01, Merck Biosciences, Nottingham, UK, 1 µg/ml (Explants), 0.1 µg/ml (BeWo)), Mdm2 (Clone 2A10, Merck Biosciences, 2 µg/ml), anti-p21 (Clone EA10, Merck Biosciences, 1∶100), Bak (TC-102, Merck Biosciences, 1∶200), Bcl-2 (Clone 100/D5, Abcam, Cambridge, UK, 1 µg/ml), Procaspase-3 (Clone 84803, R&D Systems, Abingdon, UK, 1∶1000), Procaspase 8 (Clone 84131, Merck Biosciences, 1∶100), Myosin Light Chain (Clone MY21, Abcam, 0.1 µg/ml), β-actin, (Clone AC15, Sigma, 1∶10,000) or rabbit polyclonal antibody against Bax (ab7977, Abcam, 1 µg/ml (Explants), 0.2 µg/ml (BeWo)), p21 (Abcam, 0.2 µg/ml (BeWo)), Puma (Abcam, 4 µg/ml) or β-actin (Clone AC15, Sigma, 1∶10,000).

    Techniques: Transfection, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Expressing, TUNEL Assay, Immunostaining, Electron Microscopy, Cell Culture

    ( A ) Representative Western Blots of control and Nutlin-3 treated normal placental lysates. Densitometry revealed a significant increase in the expression of ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma (*p<0.05, n = 5). ( F ) There was no effect on Bax. Co-treatment of BeWo cells with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) reduced caspase-3/7 activity ( G ) and TUNEL staining ( H ) to the level of controls. Representative images of TUNEL staining in ( I ) control, ( J ) Nutlin-3 and ( K ) co-treatment with Nutlin-3 and Pifithrin-α. Blue = DAPI, Green = TUNEL. Scale bar = 50 µm.

    Journal: PLoS ONE

    Article Title: Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast

    doi: 10.1371/journal.pone.0087621

    Figure Lengend Snippet: ( A ) Representative Western Blots of control and Nutlin-3 treated normal placental lysates. Densitometry revealed a significant increase in the expression of ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma (*p<0.05, n = 5). ( F ) There was no effect on Bax. Co-treatment of BeWo cells with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) reduced caspase-3/7 activity ( G ) and TUNEL staining ( H ) to the level of controls. Representative images of TUNEL staining in ( I ) control, ( J ) Nutlin-3 and ( K ) co-treatment with Nutlin-3 and Pifithrin-α. Blue = DAPI, Green = TUNEL. Scale bar = 50 µm.

    Article Snippet: Membranes were blocked for 1 hr with 3% (w/v) milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBS-T) and then probed overnight at 4°C, with mouse monoclonal antibodies to either p53 (Clone D01, Merck Biosciences, Nottingham, UK, 1 µg/ml (Explants), 0.1 µg/ml (BeWo)), Mdm2 (Clone 2A10, Merck Biosciences, 2 µg/ml), anti-p21 (Clone EA10, Merck Biosciences, 1∶100), Bak (TC-102, Merck Biosciences, 1∶200), Bcl-2 (Clone 100/D5, Abcam, Cambridge, UK, 1 µg/ml), Procaspase-3 (Clone 84803, R&D Systems, Abingdon, UK, 1∶1000), Procaspase 8 (Clone 84131, Merck Biosciences, 1∶100), Myosin Light Chain (Clone MY21, Abcam, 0.1 µg/ml), β-actin, (Clone AC15, Sigma, 1∶10,000) or rabbit polyclonal antibody against Bax (ab7977, Abcam, 1 µg/ml (Explants), 0.2 µg/ml (BeWo)), p21 (Abcam, 0.2 µg/ml (BeWo)), Puma (Abcam, 4 µg/ml) or β-actin (Clone AC15, Sigma, 1∶10,000).

    Techniques: Western Blot, Expressing, Activity Assay, TUNEL Assay, Staining

    ( A ) Western Blots of Nutlin-3 treated BeWo cell lysates and co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) demonstrated no effect upon ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma or ( F ) Bax protein expression (n = 5).

    Journal: PLoS ONE

    Article Title: Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast

    doi: 10.1371/journal.pone.0087621

    Figure Lengend Snippet: ( A ) Western Blots of Nutlin-3 treated BeWo cell lysates and co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) demonstrated no effect upon ( B ) p53, ( C ) Mdm2, ( D ) p21, ( E ) Puma or ( F ) Bax protein expression (n = 5).

    Article Snippet: Membranes were blocked for 1 hr with 3% (w/v) milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBS-T) and then probed overnight at 4°C, with mouse monoclonal antibodies to either p53 (Clone D01, Merck Biosciences, Nottingham, UK, 1 µg/ml (Explants), 0.1 µg/ml (BeWo)), Mdm2 (Clone 2A10, Merck Biosciences, 2 µg/ml), anti-p21 (Clone EA10, Merck Biosciences, 1∶100), Bak (TC-102, Merck Biosciences, 1∶200), Bcl-2 (Clone 100/D5, Abcam, Cambridge, UK, 1 µg/ml), Procaspase-3 (Clone 84803, R&D Systems, Abingdon, UK, 1∶1000), Procaspase 8 (Clone 84131, Merck Biosciences, 1∶100), Myosin Light Chain (Clone MY21, Abcam, 0.1 µg/ml), β-actin, (Clone AC15, Sigma, 1∶10,000) or rabbit polyclonal antibody against Bax (ab7977, Abcam, 1 µg/ml (Explants), 0.2 µg/ml (BeWo)), p21 (Abcam, 0.2 µg/ml (BeWo)), Puma (Abcam, 4 µg/ml) or β-actin (Clone AC15, Sigma, 1∶10,000).

    Techniques: Western Blot, Expressing

    Co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) had no effect on ( A ) p53, ( B ) Mdm2 or ( E ) Bax mRNA expression. Treatment with Nutlin-3 increased ( C ) p21 and ( D ) Puma expression an effect lost by co-treatment with Pifithrin-α (*p<0.05, **p<0.01, n = 5).

    Journal: PLoS ONE

    Article Title: Preeclampsia Is Associated with Alterations in the p53-Pathway in Villous Trophoblast

    doi: 10.1371/journal.pone.0087621

    Figure Lengend Snippet: Co-treatment with Nutlin-3 (30 µM) and Pifithrin-α (10 µM) had no effect on ( A ) p53, ( B ) Mdm2 or ( E ) Bax mRNA expression. Treatment with Nutlin-3 increased ( C ) p21 and ( D ) Puma expression an effect lost by co-treatment with Pifithrin-α (*p<0.05, **p<0.01, n = 5).

    Article Snippet: Membranes were blocked for 1 hr with 3% (w/v) milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBS-T) and then probed overnight at 4°C, with mouse monoclonal antibodies to either p53 (Clone D01, Merck Biosciences, Nottingham, UK, 1 µg/ml (Explants), 0.1 µg/ml (BeWo)), Mdm2 (Clone 2A10, Merck Biosciences, 2 µg/ml), anti-p21 (Clone EA10, Merck Biosciences, 1∶100), Bak (TC-102, Merck Biosciences, 1∶200), Bcl-2 (Clone 100/D5, Abcam, Cambridge, UK, 1 µg/ml), Procaspase-3 (Clone 84803, R&D Systems, Abingdon, UK, 1∶1000), Procaspase 8 (Clone 84131, Merck Biosciences, 1∶100), Myosin Light Chain (Clone MY21, Abcam, 0.1 µg/ml), β-actin, (Clone AC15, Sigma, 1∶10,000) or rabbit polyclonal antibody against Bax (ab7977, Abcam, 1 µg/ml (Explants), 0.2 µg/ml (BeWo)), p21 (Abcam, 0.2 µg/ml (BeWo)), Puma (Abcam, 4 µg/ml) or β-actin (Clone AC15, Sigma, 1∶10,000).

    Techniques: Expressing